LNNano - Brazilian Nanotechnology National Laboratory

Smartphone for point-of-care quantification of protein by Bradford assay

Camila L. de Camargo, Marcia B. R. Vicentini, Rui C. Murer, Angelo L. Gobbi,
Diego S. T. Martinez,* and Renato S. Lima*

Laboratório de Microfabricação, Laboratório Nacional de Nanotecnologia, Centro Nacional de Pesquisa em Energia e Materiais, Campinas, São Paulo 13083-100, Brasil.
*Corresponding authors. E-mails: diego.martinez@lnnano.cnpem.br & renato.lima@lnnano.cnpem.br

We address in this paper a powerful point-of-care platform to conduct the Bradford assay. Our method was based on smartphone for colorimetric quantification of total protein in human blood plasma, presenting low cost, simplicity, portability, autonomy and ability for remote transmission of the data. Other advantage concerns the high number of smartphone’s users worldwide. This feature contributes for the application of the method by non-specialist people. The interferences from external light were successfully solved by illuminating the samples with a straightforward negatoscope. Our method generated a satisfactory exactness, with accuracy percentages ranging from 87.2 to 99.1%.

This article was published in Journal of the Brazilian Chemical Society (DOI 10.21577/0103-5053.20160214). The authors would like to thank Gustavo M. Moreno (CNPEM, Campinas-SP, Brazil) for taking the photos. A video showing the analytical routine of the smartphone-based method is available at the Supplementary Information and the link: https://www.youtube.com/watch?v=hK84Z1DkIiY.

Direct link: http://jbcs.sbq.org.br/imagebank/pdf/160360SR.pdf